The nearly 90-minute test flight, which took place at Eglin Air Base in the Florida Panhandle, is part of a wide-ranging effort by the US military to reduce dependence on fossil fuels. Other flights are planned later this year.
The US imports about 70% of its oil consumption. The military is the single largest US oil consumer, making it global operations potentially vulnerable to supply disruptions.
Air Force officials say the aircraft performed normally during the test flight. The Camelina-based fuel was mixed with JP-8 jet fuel.